Detection of abscisic acid used SERS substrate based on silver coating gold nanoparticles monolayer

Yanyan Zhang; Xiaodong Li; Can Li; Shixin Li; Lanlan Li; Jiandong Hu

doi:10.1117/12.2657217

18 October 2022 Detection of abscisic acid used SERS substrate based on silver coating gold nanoparticles monolayer

Yanyan Zhang, Xiaodong Li, Can Li, Shixin Li, Lanlan Li, Jiandong Hu

Proceedings Volume 12349, International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022); 123490D (2022) https://doi.org/10.1117/12.2657217
Event: International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022), 2022, Zhengzhou, China

Abstract

Although the concentration of plant hormone abscisic acid (ABA) in crops is very low, it can effectively regulate the growth of crops. Traditional ABA detection methods are limited by expensive instruments and cumbersome sample processing. Therefore, a new method for detecting ABA in ultra-low concentrations is urgently needed. In this paper, a new method for fast and accurate determination of ABA content based on Surface Enhanced Raman Spectroscopy (SERS) was proposed. In this method, a solution of silver-coated gold nanoparticles (Au@Ag) was self-assembled into dense monolayer under the action of capillary gradient force at the air/water interface, which could be transferred to PDMS wafers and filter paper as SERS active substrates. R6G was used as a probe molecule to characterize the Sensitivity and accuracy of PDMS SERS active substrates. When the PDMS active substrate was selected to detect ABA, the detection limit 1×10−11 M was obtained, indicating that PDMS supported core-shell precious metal monolayer substrate can be used as a effective active substrate for detecting ABA. This method is also expected to be applied to the detection of other plant hormones such as corn and soybean.

Citation Download Citation

Yanyan Zhang, Xiaodong Li, Can Li, Shixin Li, Lanlan Li, and Jiandong Hu "Detection of abscisic acid used SERS substrate based on silver coating gold nanoparticles monolayer", Proc. SPIE 12349, International Conference on Agri-Photonics and Smart Agricultural Sensing Technologies (ICASAST 2022), 123490D (18 October 2022); https://doi.org/10.1117/12.2657217

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KEYWORDS

Nanoparticles

Gold

Surface enhanced Raman spectroscopy

Silver

Raman spectroscopy

Coating

Molecules

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