Cell-acquiring fallopian endoscope for detection of ovarian cancer via reflectance imaging, fluorescence imaging, and cell collection

Dominique Galvez; Ricky Cordova; Kelli Kiekens; Andrew Rocha; William Drake; Photini Rice; John M. Heusinkveld; Jennifer Barton

doi:10.1117/12.2650875

14 March 2023 Cell-acquiring fallopian endoscope for detection of ovarian cancer via reflectance imaging, fluorescence imaging, and cell collection

Dominique Galvez, Ricky Cordova, Kelli Kiekens, Andrew Rocha, William Drake, Photini Rice, John M. Heusinkveld, Jennifer Barton

Author Affiliations +

Proceedings Volume 12356, Endoscopic Microscopy XVIII; 1235603 (2023) https://doi.org/10.1117/12.2650875
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Ovarian cancer is the deadliest gynecological cancer, with most cases of high-grade serous ovarian carcinoma originating as serous tubal intraepithelial carcinoma (STIC) lesions in the fallopian tube epithelium. The Cell-Acquiring Fallopian Endoscope (CAFE) was designed to optically detect these STIC lesions and collect cells from the suspicious site for further analysis. While approximately 0.93 mm in diameter, the CAFE is able to perform multispectral fluorescence imaging (MFI), white light imaging for navigation, and cell collection. Each of these modalities is useful to locating potentially pathological areas. To find these regions, the CAFE looks for alterations of the autofluorescence of the tissue. Upon identification of a potential STIC lesion, a scrape biopsy collects cells from the region of interest. The prototype CAFE achieved an imaging resolution of 88 μm at a 5 mm distance, and 45° full field of view in air. When tested on ex vivo porcine tissue, hemocytometry counts determined that on the order of 10⁵ cells per scrape biopsy could be collected. Current progress on the CAFE includes cell collection testing on ex vivo porcine and human tissue, and improvements in the imaging resolution.

Conference Presentation

Citation Download Citation

Dominique Galvez, Ricky Cordova, Kelli Kiekens, Andrew Rocha, William Drake, Photini Rice, John M. Heusinkveld, and Jennifer Barton "Cell-acquiring fallopian endoscope for detection of ovarian cancer via reflectance imaging, fluorescence imaging, and cell collection", Proc. SPIE 12356, Endoscopic Microscopy XVIII, 1235603 (14 March 2023); https://doi.org/10.1117/12.2650875

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available