Multimodal optical elastography assessment of a murine collagen XII knockout

Achuth Nair; Yogeshwari Ambekar; Christian Zevallos-Delgado; Mei Sun; Manmohan Singh; Fernando Zvietcovich; Taye Mekonnen; Salavat Aglyamov; Manuel Koch; Giuliano Scarcelli; Edgar M. Espana; Kirill V. Larin

doi:10.1117/12.2661151

15 March 2023 Multimodal optical elastography assessment of a murine collagen XII knockout

Achuth Nair, Yogeshwari Ambekar, Christian Zevallos-Delgado, Mei Sun, Manmohan Singh, Fernando Zvietcovich, Taye Mekonnen, Salavat Aglyamov, Manuel Koch, Giuliano Scarcelli, Edgar M. Espana, Kirill V. Larin

Author Affiliations +

Proceedings Volume 12381, Optical Elastography and Tissue Biomechanics X; 123810F (2023) https://doi.org/10.1117/12.2661151
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Measuring the mechanical properties of the cornea can help understand the structure and physiology of the eye, early detection of disease, and evaluation of therapy outcomes. In this work, we investigate the effect of collagen XII deficiency on the stiffness of the murine cornea using a multimodal approach for biomechanical analysis. Wave-based optical coherence elastography (OCE), heartbeat OCE, and Brillouin microscopy were all utilized to assess the mechanical properties of wild-type and collagen XII deficient ex vivo murine corneas as a function of IOP. All three techniques show that collagen XII deficiency leads to a dramatic decrease in corneal stiffness. Future work will investigate how these measurement techniques can be translated for in vivo assessment of corneal elasticity to understand the contribution of various proteins to corneal structural and mechanical integrity.

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Achuth Nair, Yogeshwari Ambekar, Christian Zevallos-Delgado, Mei Sun, Manmohan Singh, Fernando Zvietcovich, Taye Mekonnen, Salavat Aglyamov, Manuel Koch, Giuliano Scarcelli, Edgar M. Espana, and Kirill V. Larin "Multimodal optical elastography assessment of a murine collagen XII knockout", Proc. SPIE 12381, Optical Elastography and Tissue Biomechanics X, 123810F (15 March 2023); https://doi.org/10.1117/12.2661151

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