Ultra-fast fluorescence decay in biological material

Lukas Braun; Julius Heitz; Vladislav Shcheslavskiy; Cornelia Junghans; Oksana Garanina; Vadim Elagin; Wolfgang Becker

doi:10.1117/12.2655628

25 April 2023 Ultra-fast fluorescence decay in biological material

Lukas Braun, Julius Heitz, Vladislav Shcheslavskiy, Cornelia Junghans, Oksana Garanina, Vadim Elagin, Wolfgang Becker

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 1238406 (2023) https://doi.org/10.1117/12.2655628
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Using Becker & Hickl TCSPC FLIM systems with fast TCSPC modules and fast detectors, we found ultra-fast fluorescence decay components in biological material, such as mushroom spores, pollen grains, plant tissue, and malignant melanoma. The component decay times are on the order of 10 to 50 ps, the amplitudes range from about 60% to more than 99%. In general, the shortest lifetimes and the largest amplitudes of the fast component were found in strongly coloured material, such as black mushroom spores and melanoma tissue. However, the lifetime not always correlates with the colour. In particular, a fast decay component was not found in tissue from basal cell papilloma, although it is dark brown. This may open a way to identify melanoma tissue and melanoma cells, and thus provide a new tool to investigate melanoma progression.

Conference Presentation

Citation Download Citation

Lukas Braun, Julius Heitz, Vladislav Shcheslavskiy, Cornelia Junghans, Oksana Garanina, Vadim Elagin, and Wolfgang Becker "Ultra-fast fluorescence decay in biological material", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 1238406 (25 April 2023); https://doi.org/10.1117/12.2655628

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