NIR fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Xavier Michalet; Arin C. Ulku; Michael A. Wayne; Shimon Weiss; Claudio Bruschini; Edoardo Charbon

doi:10.1117/12.2649227

25 April 2023 NIR fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera

Xavier Michalet, Arin C. Ulku, Michael A. Wayne, Shimon Weiss, Claudio Bruschini, Edoardo Charbon

Proceedings Volume 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII; 1238409 (2023) https://doi.org/10.1117/12.2649227
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

SwissSPAD3 is the latest of a family of widefield time-gated SPAD imagers developed for fluorescence lifetime imaging (FLI) applications. Its distinctive features are (i) the ability to define shorter gates than its predecessors (width W < 1 ns), (ii) support for laser repetition rates up to at least 80 MHz and (iii) a dual-gate architecture providing an effective duty cycle of 100%. We present widefield macroscopic FLI measurements of short lifetime NIR dyes, analyzed using the phasor approach. The results are compared with those previously obtained with SwissSPAD2 and to theoretical predictions.

Conference Presentation

Citation Download Citation

Xavier Michalet, Arin C. Ulku, Michael A. Wayne, Shimon Weiss, Claudio Bruschini, and Edoardo Charbon "NIR fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera", Proc. SPIE 12384, Multiphoton Microscopy in the Biomedical Sciences XXIII, 1238409 (25 April 2023); https://doi.org/10.1117/12.2649227

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