Time-resolved super-resolution microscopy to image photoluminescence lifetimes and spatially resolve dual emitter semiconductor nanostructures

Megan K. Dunlap; Liam A. Koch; Duncan P. Ryan; Peter M. Goodwin; James H. Werner; Paul B. Bourdin; Jennifer A. Hollingsworth; Chris J. Sheehan; Martin P. Gelfand; Alan Van Orden

doi:10.1117/12.2650337

15 March 2023 Time-resolved super-resolution microscopy to image photoluminescence lifetimes and spatially resolve dual emitter semiconductor nanostructures

Megan K. Dunlap, Liam A. Koch, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Paul B. Bourdin, Jennifer A. Hollingsworth, Chris J. Sheehan, Martin P. Gelfand, Alan Van Orden

Author Affiliations +

Proceedings Volume 12386, Single Molecule Spectroscopy and Superresolution Imaging XVI; 123860C (2023) https://doi.org/10.1117/12.2650337
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

A time-dependent likelihood distribution for analyzing time correlated single photon counting data from a four-pixel time-resolved single molecule localization microscopy experiment is discussed. It is generated by accounting for the probabilities to record photons from two emitters, background counts, and dark counts during two different time channels relative to each incident laser pulse in the experiment. Maximizing the distribution enables localization of each emitter in a dual emitting nanostructure based on the disparate photoluminescence lifetimes of the emitters, even when both emitters are simultaneously in an emissive state. The technique is demonstrated using simulated photon counting data from a hypothetical non-blinking dual-emitter nanostructure in which the distance between the two emitters is less than 10-nm.

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Megan K. Dunlap, Liam A. Koch, Duncan P. Ryan, Peter M. Goodwin, James H. Werner, Paul B. Bourdin, Jennifer A. Hollingsworth, Chris J. Sheehan, Martin P. Gelfand, and Alan Van Orden "Time-resolved super-resolution microscopy to image photoluminescence lifetimes and spatially resolve dual emitter semiconductor nanostructures", Proc. SPIE 12386, Single Molecule Spectroscopy and Superresolution Imaging XVI, 123860C (15 March 2023); https://doi.org/10.1117/12.2650337

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