Paper fluidic platform with dual-optical readouts to detect microRNA-20a (miR-20a) for preeclampsia

Nandita Chaturvedi; Alyssa Kunkel; Monika Schechinger; Samuel Mabbott; Mahua Choudhury; Gerard L. Coté

doi:10.1117/12.2648423

16 March 2023 Paper fluidic platform with dual-optical readouts to detect microRNA-20a (miR-20a) for preeclampsia

Nandita Chaturvedi, Alyssa Kunkel, Monika Schechinger, Samuel Mabbott, Mahua Choudhury, Gerard L. Coté

Proceedings Volume 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics; 123870G (2023) https://doi.org/10.1117/12.2648423
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Preeclampsia (PE) is a condition that affects women during pregnancy. It is a leading cause of maternal and infant death worldwide and is typically detected in the second trimester or later. Predicting PE at an early stage is one of the most important goals of maternal-fetal medicine. Thus, there is an unmet need for accurate early detection of preeclampsia, ideally at the point-of-care. In a pilot study, microRNA-20a (miR-20a) showed an upregulation in the blood samples of the first trimester of preeclamptic pregnancies when compared to healthy mothers. In this research, a dual capture probe sandwich assay, sensitive to miR-20a, that used surface-enhanced Raman scattering (SERS)-active gold nanoparticles for the signal transduction was developed. The assay was translated to a lateral flow paper fluidic device, for potential low cost and ease of use in low-resource settings. Each paper layer has different pore sizes and features an array of buffers that store nanoparticle conjugates, guide flow, and reduce non-specific binding. Using a handheld Raman reader with a 638 nm excitation laser, reliable SERS detection of miR-20a was shown with resolutions down to 1 nM. Colorimetric detection of these concentrations using the RGB pixels from scanning the test line is also depicted, showing multi-modal detection to potentially enhance specificity for this early epigenetic biomarker.

Conference Presentation

Citation Download Citation

Nandita Chaturvedi, Alyssa Kunkel, Monika Schechinger, Samuel Mabbott, Mahua Choudhury, and Gerard L. Coté "Paper fluidic platform with dual-optical readouts to detect microRNA-20a (miR-20a) for preeclampsia", Proc. SPIE 12387, Optical Diagnostics and Sensing XXIII: Toward Point-of-Care Diagnostics, 123870G (16 March 2023); https://doi.org/10.1117/12.2648423

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available