Toward the specificity in QPI 3D tomographic cell flow cytometry holography: recent achievements and perspectives in biomedical sciences

Daniele Pirone; Joowon Lim; Francesco Merola; Lisa Miccio; Martina Mugnano; Vittorio Bianco; Marika Valentino; Giusy Giugliano; Flora Cimmino; Feliciano Visconte; Annalaura Montella; Mario Capasso; Achille Iolascon; Pasquale Memmolo; Demetri Psaltis; Pietro Ferraro

doi:10.1117/12.2665523

16 March 2023 Toward the specificity in QPI 3D tomographic cell flow cytometry holography: recent achievements and perspectives in biomedical sciences

Daniele Pirone, Joowon Lim, Francesco Merola, Lisa Miccio, Martina Mugnano, Vittorio Bianco, Marika Valentino, Giusy Giugliano, Flora Cimmino, Feliciano Visconte, Annalaura Montella, Mario Capasso, Achille Iolascon, Pasquale Memmolo, Demetri Psaltis, Pietro Ferraro

Author Affiliations +

Proceedings Volume 12389, Quantitative Phase Imaging IX; 1238902 (2023) https://doi.org/10.1117/12.2665523
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

The actual gap of the label-free quantitative phase microscopy in respect to fluorescence microscopy, that allows the subcellular characterization by using exogenous markers, is the lack of intracellular specificity. Recently, computational methods based on artificial intelligence have been demonstrated, which allow a virtual staining of single cells in both 2D and 3D, but they require co-registration systems able to collect simultaneously both fluorescence and quantitative phase information. However, a real limitation exists, i.e. these approaches cannot be used in flow cytometry condition. In this paper, we discuss a new methodology for adding the intracellular specificity analysis to tomographic phase microscopy in flow cytometry. The proposed strategy is based on the statistical clustering of tomograms voxels, thus allowing the segmentation of cell’s organelles. Here we report the results of nuclear region identification for cancer cells.

Conference Presentation

Citation Download Citation

Daniele Pirone, Joowon Lim, Francesco Merola, Lisa Miccio, Martina Mugnano, Vittorio Bianco, Marika Valentino, Giusy Giugliano, Flora Cimmino, Feliciano Visconte, Annalaura Montella, Mario Capasso, Achille Iolascon, Pasquale Memmolo, Demetri Psaltis, and Pietro Ferraro "Toward the specificity in QPI 3D tomographic cell flow cytometry holography: recent achievements and perspectives in biomedical sciences", Proc. SPIE 12389, Quantitative Phase Imaging IX, 1238902 (16 March 2023); https://doi.org/10.1117/12.2665523

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available