Label-free 3D visualization and quantification of endogenous and exogenous intracellular particles in single cells by holographic flow tomography

Daniele Pirone; Martina Mugnano; Daniele Sirico; Lisa Miccio; Vittorio Bianco; Francesco Merola; Danila del Giudice; Giuseppe Cesare Lama; Rachele Castaldo; Simonetta Grilli; Giannandrea Pasquinelli; Sabrina Valente; Silvia Lemma; Luisa Iommarini; Ivana Kurelac; Pasquale Memmolo; Pietro Ferraro

doi:10.1117/12.2663945

16 March 2023 Label-free 3D visualization and quantification of endogenous and exogenous intracellular particles in single cells by holographic flow tomography

Daniele Pirone, Martina Mugnano, Daniele Sirico, Lisa Miccio, Vittorio Bianco, Francesco Merola, Danila del Giudice, Giuseppe Cesare Lama, Rachele Castaldo, Simonetta Grilli, Giannandrea Pasquinelli, Sabrina Valente, Silvia Lemma, Luisa Iommarini, Ivana Kurelac, Pasquale Memmolo, Pietro Ferraro

Author Affiliations +

Proceedings Volume 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023; 123910B (2023) https://doi.org/10.1117/12.2663945
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Detection and quantification of intracellular structures is fundamental in biomedical sciences. New emerging inspection tools based on holographic microscopy and quantitative phase imaging can give answers to such critical demands. Holographic tomography (HT) systems are the best candidates for this challenge. Recently, HT has been demonstrated working in flow-cytometry (FC) modality. Results show that the novel HTFC tool is capable to furnish 3D visualization and quantifications of the different intracellular particles. In particular, here we report that exogenous nanographene oxide particles as well as endogenous lipid droplets can be detected, measured, and visualized in each flowing cell by label-free HTFC. This method opens the way for accurate and high-throughput measurements at the 3D single-cell level for different applications such as diagnosis of diseases, development of drug delivery applications, and examination of cell functionalities. Experiments and processing methods will be described, and several examples will be discussed.

Conference Presentation

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Daniele Pirone, Martina Mugnano, Daniele Sirico, Lisa Miccio, Vittorio Bianco, Francesco Merola, Danila del Giudice, Giuseppe Cesare Lama, Rachele Castaldo, Simonetta Grilli, Giannandrea Pasquinelli, Sabrina Valente, Silvia Lemma, Luisa Iommarini, Ivana Kurelac, Pasquale Memmolo, and Pietro Ferraro "Label-free 3D visualization and quantification of endogenous and exogenous intracellular particles in single cells by holographic flow tomography", Proc. SPIE 12391, Label-free Biomedical Imaging and Sensing (LBIS) 2023, 123910B (16 March 2023); https://doi.org/10.1117/12.2663945

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