Widefield mid-infrared photothermal heterodyne imaging in the cell-silent window

Eduardo M. Paiva; Florian M. Schmidt

doi:10.1117/12.2654181

15 March 2023 Widefield mid-infrared photothermal heterodyne imaging in the cell-silent window

Proceedings Volume 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; 1239207 (2023) https://doi.org/10.1117/12.2654181
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Widefield mid-infrared photothermal heterodyne (WIPH) microscopy enables sensitive and fast chemical imaging with high spatial resolution. The technique is realized using an external-cavity quantum cascade laser emitting and a digital frequency-domain lock-in filter for simultaneous multi-harmonic demodulation of WIPH signals recorded by individual camera pixels at a frame rate of 20 kHz. The filter allows the use of continuous-wave probe light and the time-resolved detection of photothermal decay curves. The microscope provides <1 µm spatial resolution in a 64x64 µm field of view. Here, we present preliminary results from hyperspectral WIPH imaging of alkyne-tagged palmitic acid (PA), azidetagged PA and perdeuterated PA via their absorption features in the cell-silent spectral region around 2100 cm^-1. The alkyne and azide functional groups and deuterium are promising vibrational probes for selective imaging of biomolecules, such as lipids and proteins, in cells.

Conference Presentation

Citation Download Citation

Eduardo M. Paiva and Florian M. Schmidt "Widefield mid-infrared photothermal heterodyne imaging in the cell-silent window", Proc. SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, 1239207 (15 March 2023); https://doi.org/10.1117/12.2654181

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