Detection of Bacillus group spores in an aqueous environment using an aptamer based digital photocorrosion biosensor

Ishika Ishika; Walid M. Hassen; Houman Moteshareie; Azam F. Tayabali; Jan J. Dubowski

doi:10.1117/12.2662421

15 March 2023 Detection of Bacillus group spores in an aqueous environment using an aptamer based digital photocorrosion biosensor

Ishika Ishika, Walid M. Hassen, Houman Moteshareie, Azam F. Tayabali, Jan J. Dubowski

Author Affiliations +

Proceedings Volume 12410, Nanoscale and Quantum Materials: From Synthesis and Laser Processing to Applications 2023; 1241003 (2023) https://doi.org/10.1117/12.2662421
Event: SPIE LASE, 2023, San Francisco, California, United States

Abstract

Bacillus cereus (Bc) group are spore-forming bacteria that include human pathogenic strains, such as Bacillus cereus and Bacillus anthracis. Detection of Bc group spores using selective media is a laborious process that is largely dependent on the laboratory environment. We have examined the application of so-called digital photocorrosion (DIP) GaAs/AlGaAs biosensor for detection of Bacillus thuringiensis (Bt) spores. With the aptamer-based biosensing architecture, we have successfully demonstrated detection of Bt spores in the range between 10³ - 10⁵ spores/mL. The ease of operating DIP biosensors, and the potential for sensitive detection in field settings hold the promise of attractive applications of these innovative devices for monitoring the presence of Bc group spores.

Conference Presentation

Citation Download Citation

Ishika Ishika, Walid M. Hassen, Houman Moteshareie, Azam F. Tayabali, and Jan J. Dubowski "Detection of Bacillus group spores in an aqueous environment using an aptamer based digital photocorrosion biosensor", Proc. SPIE 12410, Nanoscale and Quantum Materials: From Synthesis and Laser Processing to Applications 2023, 1241003 (15 March 2023); https://doi.org/10.1117/12.2662421

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