Nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection

Eun-Sil Yu; Byoung-Hoon Kang; Hamin Na; Ki-Hun Jeong

doi:10.1117/12.2650879

17 March 2023 Nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection

Eun-Sil Yu, Byoung-Hoon Kang, Hamin Na, Ki-Hun Jeong

Proceedings Volume 12434, MOEMS and Miniaturized Systems XXII; 124340D (2023) https://doi.org/10.1117/12.2650879
Event: SPIE OPTO, 2023, San Francisco, California, United States

Abstract

This paper reports a nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection. The study utilizes a microchip and a miniaturized hand-held type photothermal PCR system, which comprises a nanoplasmonic photothermal heater and microlens array camera to maintain the temperature and detect fluorescence signal from the chip. CRISPR-based fluorescence signal detection, which proceeds simultaneously with nucleic acid amplification, indicates higher sensitivity and rapid detection. The real-time nanoplasmonic isothermal PCR assay opens a new opportunity for POCT-based CRISPR assay.

Conference Presentation

Citation Download Citation

Eun-Sil Yu, Byoung-Hoon Kang, Hamin Na, and Ki-Hun Jeong "Nanoplasmonic isothermal PCR assay with CRISPR/Cas for real-time SARS-CoV-2 detection", Proc. SPIE 12434, MOEMS and Miniaturized Systems XXII, 124340D (17 March 2023); https://doi.org/10.1117/12.2650879

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