Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy

Pekka E. Hanninen; Juha Salo; Ernst H.K. Stelzer

doi:10.1117/12.19543

1 May 1990 Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy

Pekka E. Hanninen, Juha Salo, Ernst H.K. Stelzer

Author Affiliations +

Proceedings Volume 1245, Biomedical Image Processing; (1990) https://doi.org/10.1117/12.19543
Event: Electronic Imaging: Advanced Devices and Systems, 1990, Santa Clara, CA, United States

Abstract

In this paper a different way of processing fluorescence images with median type filters is presented. The use of linear median hybrid filtering techniques in fluorescence microscopy is shown to be very promising in trying to achieve both increased recording speed and decreased photo damage to the biological sample. It is also shown that these linear median filtering methods can tuned for both confocal and conventional fluorescence microscopy. The parallelization of such a process is discussed.

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Pekka E. Hanninen, Juha Salo, and Ernst H.K. Stelzer "Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy", Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); https://doi.org/10.1117/12.19543

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