Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy

Pekka E. Hanninen; Juha Salo; Ernst H.K. Stelzer

doi:10.1117/12.19543

1 May 1990 Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy

Pekka E. Hanninen, Juha Salo, Ernst H.K. Stelzer

Author Affiliations +

Proceedings Volume 1245, Biomedical Image Processing; (1990) https://doi.org/10.1117/12.19543
Event: Electronic Imaging: Advanced Devices and Systems, 1990, Santa Clara, CA, United States

Abstract

In this paper a different way of processing fluorescence images with median type filters is presented. The use of linear median hybrid filtering techniques in fluorescence microscopy is shown to be very promising in trying to achieve both increased recording speed and decreased photo damage to the biological sample. It is also shown that these linear median filtering methods can tuned for both confocal and conventional fluorescence microscopy. The parallelization of such a process is discussed.

Citation Download Citation

Pekka E. Hanninen, Juha Salo, and Ernst H.K. Stelzer "Nonlinear filtering methods for improving the image quality in conventional and confocal fluorescence microscopy", Proc. SPIE 1245, Biomedical Image Processing, (1 May 1990); https://doi.org/10.1117/12.19543

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

;

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE