Using FLIM to reveal acute responses to high salinity in Chlorella sp.

Zeyu Xiao; Sihan Wu; Zhen Lu; Yinchu Wang; Yifeng Deng; Wei Sun; Haipeng Liu; Yijia Zeng; Huifeng Wu; Xiao Peng; Junle Qu

doi:10.1117/12.2654855

15 December 2022 Using FLIM to reveal acute responses to high salinity in Chlorella sp.

Zeyu Xiao, Sihan Wu, Zhen Lu, Yinchu Wang, Yifeng Deng, Wei Sun, Haipeng Liu, Yijia Zeng, Huifeng Wu, Xiao Peng, Junle Qu

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Proceedings Volume 12478, Thirteenth International Conference on Information Optics and Photonics (CIOP 2022); 124783P (2022) https://doi.org/10.1117/12.2654855
Event: Thirteenth International Conference on Information Optics and Photonics (CIOP 2022), 2022, Xi'an, China

Abstract

Chlorella is a single-celled blue-green spherical microalga, whose color could change from green to red or yellowish due to the components of different types of innate pigments. Salinity change is one important environmental stressor that may influence the pigment composition of Chlorella. Therefore, it is necessary to monitor the salinity stress on Chlorella in a real-time mode. Recently, fluorescence lifetime imaging microscopy (FLIM) technology has been widely applied into biological fields, which could provide fluorescence lifetime values for quantitative analysis. Here, we used FLIM method to investigate a freshwater microalga, Chlorella sp. based on its autofluorescence. Chlorella cells were treated with a series of salinity concentrations (control sample in normal culture medium, 3S sample with an additional 3× salinity, 7S sample with an additional 7× salinity, respectively) for one day. Then images of the microalgae cells from each group were obtained with FLIM system and analyzed with SPCImage software, providing the fluorescence lifetime data. The results of fluorescence lifetime data showed that 3× salinity condition had little effect on Chlorella, which indicated that Chlorella had a strong adaptive capacity in environments close to seawater salinity. However, the significant left shift of lifetime distribution peak and decreased mean lifetime values were observed in 7S samples compared with the control. In conclusion, FLIM method has shown great potential as a fast identification method of living Chlorella sp. under high salinity conditions in a quantitative and non-invasive way.

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Zeyu Xiao, Sihan Wu, Zhen Lu, Yinchu Wang, Yifeng Deng, Wei Sun, Haipeng Liu, Yijia Zeng, Huifeng Wu, Xiao Peng, and Junle Qu "Using FLIM to reveal acute responses to high salinity in Chlorella sp.", Proc. SPIE 12478, Thirteenth International Conference on Information Optics and Photonics (CIOP 2022), 124783P (15 December 2022); https://doi.org/10.1117/12.2654855

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KEYWORDS

Luminescence

Fluorescence lifetime imaging

Microscopy

Confocal microscopy

Molecular lasers

Analytical research

Molecules

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