Signatures of irradiated cells from hyperspectral images

Raluca D. Negoita; Mihaela A. Ungureanu; Roxana C. Popescu; Ana M. Pleava; Mihaela Tudor; Anca Dinischiotu; Diana Savu; Mona Mihailescu; Eugen N. Scarlat

doi:10.1117/12.2642654

2 March 2023 Signatures of irradiated cells from hyperspectral images

Raluca D. Negoita, Mihaela A. Ungureanu, Roxana C. Popescu, Ana M. Pleava, Mihaela Tudor, Anca Dinischiotu, Diana Savu, Mona Mihailescu, Eugen N. Scarlat

Author Affiliations +

Proceedings Volume 12493, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies XI; 124932K (2023) https://doi.org/10.1117/12.2642654
Event: Advanced Topics in Optoelectronics, Microelectronics and Nanotechnologies 2022, 2022, Constanta, Romania

Abstract

We used enhanced darkfield microscopy (EDM) to record hyperspectral images (HSI) of attached cells from SW1353 chondrosarcoma cell line exposed to ionizing radiation (X-rays, protons). Our investigations are conducted to highlight the effects induced by the incident radiation onto cells characteristics, their structural and morphological changes, revealed by HSI images. The cell nucleus was segmented and analysed separately on their entire area or at the level of a single pixel. Global analysis of the whole nucleus area was performed to find geometrical parameters: area, perimeter, major axis, circularity, eccentricity, solidity. Statistical analysis was performed on distributions of intensity values separated on five spectral intervals. Local analysis, at single-pixel level or at regions of interest was performed in terms of spectral profile, separated on nucleus and cytoplasm. This study suggests that the nonirradiated cells have spectral profiles with multiple maxima, in contrast with the irradiated cells which display single-peak spectral profiles. Differences between irradiated and nonirradiated cells were observed in parameters like area, eccentricity, solidity, kurtosis, skewness at different spectral intervals.

Citation Download Citation

Raluca D. Negoita, Mihaela A. Ungureanu, Roxana C. Popescu, Ana M. Pleava, Mihaela Tudor, Anca Dinischiotu, Diana Savu, Mona Mihailescu, and Eugen N. Scarlat "Signatures of irradiated cells from hyperspectral images", Proc. SPIE 12493, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies XI, 124932K (2 March 2023); https://doi.org/10.1117/12.2642654

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Statistical analysis

Hyperspectral imaging

Microscopy

Image segmentation

Ionizing radiation

Nanoparticles

Tissues

Show All Keywords

Keywords/Phrases

Search In:

Publication Years