Smartphone-based pathogen detection using simultaneous monitoring of resonant frequency and optical fluorescence

Hyun Jung Min; Hansel A. Mina; Amanda J. Deering; Bartek Rajwa; Sungho Shin; Iyll-Joon Doh; J. Paul Robinson; Euiwon Bae

doi:10.1117/12.2665235

13 June 2023 Smartphone-based pathogen detection using simultaneous monitoring of resonant frequency and optical fluorescence

Hyun Jung Min, Hansel A. Mina, Amanda J. Deering, Bartek Rajwa, Sungho Shin, Iyll-Joon Doh, J. Paul Robinson, Euiwon Bae

Author Affiliations +

Proceedings Volume 12545, Sensing for Agriculture and Food Quality and Safety XV; 125450A (2023) https://doi.org/10.1117/12.2665235
Event: SPIE Defense + Commercial Sensing, 2023, Orlando, Florida, United States

Abstract

Recently, the use of a Quartz Crystal Microbalance (QCM) as a biosensor for detecting foodborne pathogens by observing changes in resonant frequency has gained popularity. However, conventional detection methods are time-consuming and require expensive equipment and trained personnel. The current trend is toward detection approaches that are quick, portable, and easy to use. In order to address this need, a dual-modality QCM system combining a smartphone, an in-situ fluorescence imaging subsystem, and a flow injection component has been proposed. This system enables a smartphone to receive real-time frequency data via Bluetooth, while a camera detects the presence of bacteria on the quartz crystal surface using a fluorescence-tagged antibody. The fluorescence imaging subsystem utilizes a camera to capture the bacteria fluorescence signal, while the flow injection subsystem employs a mini peristaltic pump and controller to introduce biochemical solutions, antibodies, and bacteria. All components are contained in a 3D cartridge that is portable. FITC images were captured with 5 MHz quartz crystals when the prototype system was tested. The developed QCM biosensor has the potential to become a portable bacteria detection approach that outperforms existing techniques.

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Citation Download Citation

Hyun Jung Min, Hansel A. Mina, Amanda J. Deering, Bartek Rajwa, Sungho Shin, Iyll-Joon Doh, J. Paul Robinson, and Euiwon Bae "Smartphone-based pathogen detection using simultaneous monitoring of resonant frequency and optical fluorescence", Proc. SPIE 12545, Sensing for Agriculture and Food Quality and Safety XV, 125450A (13 June 2023); https://doi.org/10.1117/12.2665235

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