Grating-based common-path quantitative phase microscopy in low photon budget regime

M. Trusiak; P. Zdańkowski; M. Rogalski; K. Patorski

doi:10.1117/12.2665601

31 May 2023 Grating-based common-path quantitative phase microscopy in low photon budget regime

M. Trusiak, P. Zdańkowski, M. Rogalski, K. Patorski

Proceedings Volume 12574, Holography: Advances and Modern Trends VIII; 125740Y (2023) https://doi.org/10.1117/12.2665601
Event: SPIE Optics + Optoelectronics, 2023, Prague, Czech Republic

Abstract

Quantitative phase microscopy (QPM) is making waves in live cell imaging owing to label-free time-lapse investigation capabilities. Common-path straightforward configurations are advantageous because of their robustness and stability. Diffraction grating based quantitative phase microscope is a good example of such systems. Grating is employed to decouple conjugate object beams, and their total shear interference is recorded by the camera as a self-referenced hologram (object replica interferes with the object-free background replica; optical path difference between +1 and -1 orders is 0). This allows for the possibility of altering the temporal coherence and suppressing coherent noise (speckle) and artifacts. Generally, in QPM, laser light is used to generate a hologram with encoded sample phase information, and live cells can be impaired by elongated interactions with radiation. To limit the possible photo-damage and photostimulation and examine live unimpaired cells in their natural photo-stress-free environment, a low dose of radiation is deployed. In such a low photon budget regime, the signal-to-noise ratio of the recorded hologram can be drastically reduced, which leads to a strong shot-noise presence in demodulated phase maps and deteriorated the quantitative characterization and diagnosis capabilities. In this contribution, we investigate how a low photon budget affects the quantitative examination of phase objects in grating-based common-path QPM. We explore numerical methods to reduce phase noise via additional holograms and phase map filtering.

Conference Presentation

Citation Download Citation

M. Trusiak, P. Zdańkowski, M. Rogalski, and K. Patorski "Grating-based common-path quantitative phase microscopy in low photon budget regime", Proc. SPIE 12574, Holography: Advances and Modern Trends VIII, 125740Y (31 May 2023); https://doi.org/10.1117/12.2665601

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