Optimizing the experimental layout and the computational processing for lab-on-chip-based tomographic flow cytometry system

Francesca Borrelli; Jaromir Behal; Martina Mugnano; Vittorio Bianco; Amedeo Capozzoli; Claudio Curcio; Angelo Liseno; Lisa Miccio; Pasquale Memmolo; Pietro Ferraro

doi:10.1117/12.2674878

11 August 2023 Optimizing the experimental layout and the computational processing for lab-on-chip-based tomographic flow cytometry system

Francesca Borrelli, Jaromir Behal, Martina Mugnano, Vittorio Bianco, Amedeo Capozzoli, Claudio Curcio, Angelo Liseno, Lisa Miccio, Pasquale Memmolo, Pietro Ferraro

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 1262205 (2023) https://doi.org/10.1117/12.2674878
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

A quasi-common-path lateral-shearing holo-tomographic optical setup coupled to microfluidic channel is designed and build-up. We demonstrate that 3D refractive index profile of flowing cells can be efficiently retrieved. This setup is robust to external vibrations and is scaled down in dimension, in the perspective of employing such systems outside of optical labs. We apply a reconstruction algorithm based on high-order total-variation to cope with the reduced quality of the measurements acquired. Validation of such reconstruction approach is performed. The assessment of the robustness of the proposed solution is allowed by evaluating some biophysical parameters of biological relevance over the reconstructed tomograms.

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Citation Download Citation

Francesca Borrelli, Jaromir Behal, Martina Mugnano, Vittorio Bianco, Amedeo Capozzoli, Claudio Curcio, Angelo Liseno, Lisa Miccio, Pasquale Memmolo, and Pietro Ferraro "Optimizing the experimental layout and the computational processing for lab-on-chip-based tomographic flow cytometry system", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 1262205 (11 August 2023); https://doi.org/10.1117/12.2674878

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