Label-free imaging and microfluidics to diagnose space-induced effects on blood components

Lisa Miccio; Jaromir Behal; Daniele Pirone; Pasquale Memmolo; Vittorio Bianco; Martina Mugnano; Massimiliano M. Villone; Francesca Ferranti; Silvia Mari; Pier Luca Maffettone

doi:10.1117/12.2675596

11 August 2023 Label-free imaging and microfluidics to diagnose space-induced effects on blood components

Lisa Miccio, Jaromir Behal, Daniele Pirone, Pasquale Memmolo, Vittorio Bianco, Martina Mugnano, Massimiliano M. Villone, Francesca Ferranti, Silvia Mari, Pier Luca Maffettone

Author Affiliations +

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 126220J (2023) https://doi.org/10.1117/12.2675596
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

Human health and disease prevention are among the priorities to safeguard astronauts and, in the next future, space tourists. There is a great demand of new reliable biotechnologies that would be eventually implemented on spacecrafts to observe the space-induced effect on humans. One of the main risks is related to the radiation exposure, that is significantly higher than on Earth. For this reason, space agencies are pushing to develop strategies to quantify, oversee and limiting such risks. Here we present an approach based on the combination of microfluidics and stain-free imaging also aided by artificial intelligence to monitor the effect on ionizing radiation on blood cells. The system is based on the Holographic Image Flow Cytometry system where Quantitative Phase Contrast images are retrieved for cell flowing and rotating into a microfluidics circuits. Proof of concept is demonstrated where morphological parameters are identified able to distinguish cell population irradiated at different radiation doses and at different time from the radiation exposure. Blood cell will be analyzed. The presented approach has main advantages respect to standard and already existent technologies for single cell analysis. The first one is the no-need of fluorescence staining thus opening to faster and easier operation steps. The second one is related to cell rotation into the field of view, allowing to acquire images at different rotation angle and thus collecting a broader dataset useful for the application of artificial intelligence network. Furthermore, the system can be miniaturized to a scale portable out of the laboratory environment.

Conference Presentation

Citation Download Citation

Lisa Miccio, Jaromir Behal, Daniele Pirone, Pasquale Memmolo, Vittorio Bianco, Martina Mugnano, Massimiliano M. Villone, Francesca Ferranti, Silvia Mari, and Pier Luca Maffettone "Label-free imaging and microfluidics to diagnose space-induced effects on blood components", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 126220J (11 August 2023); https://doi.org/10.1117/12.2675596

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