Three-dimensional holographic particle tracking in space-time digital holography

Zhengzhong Huang; Zhe Wang; Daniele Pirone; Vittorio Bianco; Pasquale Memmolo; Liangcai Cao; Pietro Ferraro

doi:10.1117/12.2675460

11 August 2023 Three-dimensional holographic particle tracking in space-time digital holography

Zhengzhong Huang, Zhe Wang, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Liangcai Cao, Pietro Ferraro

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 126220T (2023) https://doi.org/10.1117/12.2675460
Event: SPIE Optical Metrology, 2023, Munich, Germany

Abstract

Lab-on-a-Chip microfluidic devices represent an innovative and cost-effective solution in the current trend of miniaturization and simplification of imaging flow cytometry. Cell tracking is a fundamental technique for investigating a variety of biophysical processes, from intracellular dynamics to the characterization of cell motility and migration. The conventional target positioning based on holography is typically addressed by decoupling the calculation of the optical axis position and the transverse coordinates. The 2D positions of each cell are located based on the phase contrast. The axial position of the cell area is calculated by refocused external criterion in complex amplitude wavefront. Computing resources and time consumption may increase because all the frames need to be performed calculations in the spatial frequency domain. We proposed a space-time digital hologram encoding method to speed up 3D holographic particle tracking. The 2D positions of each cell are directly located by morphological calculation based on the hologram. The complex amplitude wavefronts are directly reconstructed by space-time phase shifting to calculate the axial position by refocused external criterion. Only spatial calculation is considered in the proposed method. The proposed approach can be used in microfluidics to analyze objects flowing in microfluidics channels.

Conference Presentation

Citation Download Citation

Zhengzhong Huang, Zhe Wang, Daniele Pirone, Vittorio Bianco, Pasquale Memmolo, Liangcai Cao, and Pietro Ferraro "Three-dimensional holographic particle tracking in space-time digital holography", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 126220T (11 August 2023); https://doi.org/10.1117/12.2675460

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