Motility-based screening of probiotic bacteria candidates by label-free coherent light tester

Vittorio Bianco; Filomena Nazzaro; Zhe Wang; Jaromir Behal; Simonetta Grilli; Pietro Ferraro

doi:10.1117/12.2674359

11 August 2023 Motility-based screening of probiotic bacteria candidates by label-free coherent light tester

Vittorio Bianco, Filomena Nazzaro, Zhe Wang, Jaromir Behal, Simonetta Grilli, Pietro Ferraro

Proceedings Volume 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI; 1262216 (2023) https://doi.org/10.1117/12.2674359
Event: SPIE Optical Metrology, 2023, Munich, Germany

Conference Poster

Abstract

Probiotic bacteria are microbial species known to confer benefits to health. In order to act effectively as probiotics, microencapsulated bacteria have to maintain their viability during the gastro-intestinal transit and their motility to reach epithelial cells of the intestine. Here we use Bio-Speckle Dynamic Assays (BSDA) for rapidly testing the microencapsulation performance in experiments simulating gastro-intestinal conditions. Label-free samples are probed by coherent light to infer ensemble motility information. Then, we use Digital Holography (DH) in transmission microscopy mode and 3D tracking as complementary tools to infer strain-specific locomotion profiles at the single cell level.

Citation Download Citation

Vittorio Bianco, Filomena Nazzaro, Zhe Wang, Jaromir Behal, Simonetta Grilli, and Pietro Ferraro "Motility-based screening of probiotic bacteria candidates by label-free coherent light tester", Proc. SPIE 12622, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials VI, 1262216 (11 August 2023); https://doi.org/10.1117/12.2674359

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