Spatial reorganization of F-actin in respiratory cells as measured by Brillouin microscopy

H. Mahmodi; P. Bradbury; A. Cidem; H. X. Ong; D. Traini; I. Kabakova

doi:10.1117/12.2672413

11 August 2023 Spatial reorganization of F-actin in respiratory cells as measured by Brillouin microscopy

H. Mahmodi, P. Bradbury, A. Cidem, H. X. Ong, D. Traini, I. Kabakova

Author Affiliations +

Proceedings Volume 12629, Emerging Technologies for Cell and Tissue Characterization II; 126290O (2023) https://doi.org/10.1117/12.2672413
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Brillouin microscopy has emerged as a non-invasive and label-free technique to map micro-mechanical properties of cells. Here we apply Brillouin microscopy to probe reorganization of F-actin network in respiratory cells treated with Timothy grass pollen protein extracts. The results of our measurements in conjunction with clustering data analysis confirm spatial cellular reorganization of F-actin proteins and compromised junctional integrity in treated cells as compared to controls.

Citation Download Citation

H. Mahmodi, P. Bradbury, A. Cidem, H. X. Ong, D. Traini, and I. Kabakova "Spatial reorganization of F-actin in respiratory cells as measured by Brillouin microscopy", Proc. SPIE 12629, Emerging Technologies for Cell and Tissue Characterization II, 126290O (11 August 2023); https://doi.org/10.1117/12.2672413

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