Time-shared optical tweezers for active microrheology inside cells

Frederic Català-Castro; Paolo Antonio Frigeri; Santiago Ortiz-Vásquez; Carmen Martínez-Fernández; Michael Krieg

doi:10.1117/12.2681989

5 October 2023 Time-shared optical tweezers for active microrheology inside cells

Frederic Català-Castro, Paolo Antonio Frigeri, Santiago Ortiz-Vásquez, Carmen Martínez-Fernández, Michael Krieg

Proceedings Volume 12649, Optical Trapping and Optical Micromanipulation XX; 126490N (2023) https://doi.org/10.1117/12.2681989
Event: SPIE Nanoscience + Engineering, 2023, San Diego, California, United States

Abstract

The use of non-invasive, optical tweezers active microrheology provides invaluable information on the mechanobiological principles that govern most cellular processes. The principle of using a single laser beam to trap —either endogenous droplets or microinjected probe beads— and measure both the displacement and the force during an imposed oscillation has been proven insufficient for obtaining the response function, ˆχ(ω), and the G modulus, ˆG(ω). As a solution, an additional laser with very low power can be used to measure probe displacements independently, to the detriment of the simplicity of the optical trapping set-up, robustness and cost. Here, we present a method to carry out position and force measurements with a single trapping beam through the time-sharing mode of an optical micromanipulation unit modulated with acousto-optic deflectors.

Conference Presentation

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Frederic Català-Castro, Paolo Antonio Frigeri, Santiago Ortiz-Vásquez, Carmen Martínez-Fernández, and Michael Krieg "Time-shared optical tweezers for active microrheology inside cells", Proc. SPIE 12649, Optical Trapping and Optical Micromanipulation XX, 126490N (5 October 2023); https://doi.org/10.1117/12.2681989

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