Aberration-free high-bandwidth holographic imaging

Zhengzhong Huang; Liangcai Cao

doi:10.1117/12.2686597

4 October 2023 Aberration-free high-bandwidth holographic imaging

Zhengzhong Huang, Liangcai Cao

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Proceedings Volume 12746, SPIE-CLP Conference on Advanced Photonics 2023; 1274609 (2023) https://doi.org/10.1117/12.2686597
Event: SPIE-CLP Conference on Advanced Photonics, 2023, San Diego, California, United States

Conference Poster

Abstract

Objective measurements of the morphology and dynamics of label-free cells and tissues can be achieved by quantitative phase with low phototoxicity and no photobleaching. Modern quantitative optical imaging possesses a huge information capacity. The morphology and dynamics of label-free tissues can be exploited by sample-induced changes in the optical field from quantitative phase imaging. Its sensitivity to subtle changes in the optical field makes the reconstructed phase susceptible to phase aberrations. We present aberration-free high-bandwidth holographic microscopy which exploits high-throughput label-free quantitative phase imaging. Firstly, a full-bandwidth holographic reconstruction is retrieved from interferograms by establishing a holographic multiplexing framework. Based on the analyticity of band-limited signal under a diffraction-limited system, the maximum space bandwidth utilization limit in a single multiplexing hologram is increased to the maximum sensor limit. Secondly, A variable sparse splitting framework on quantitative phase aberration extraction is imported based on the alternating direction aberration-free method. By formulating the aberration extraction as a convex quadratic problem, the background phase aberration can be fast and directly decomposed with the specific complete basis functions such as Zernike or standard polynomials. Faithful high throughput phase reconstruction can be obtained by eliminating global background aberration. It opens a new route to multiplexing quantitative optical imaging and helps to improve the performance of constraint-free modern optical microscopes in various spectral regimes.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

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Zhengzhong Huang and Liangcai Cao "Aberration-free high-bandwidth holographic imaging", Proc. SPIE 12746, SPIE-CLP Conference on Advanced Photonics 2023, 1274609 (4 October 2023); https://doi.org/10.1117/12.2686597

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