Research on low-cost and fast frequency-shift super-resolution microscopic imaging

Qianwei Zhang; Feihong Lin; Xiaoyu Yang; Mingwei Tang; Qing Yang; Xu Liu

doi:10.1117/12.2687083

29 November 2023 Research on low-cost and fast frequency-shift super-resolution microscopic imaging

Qianwei Zhang, Feihong Lin, Xiaoyu Yang, Mingwei Tang, Qing Yang, Xu Liu

Proceedings Volume 12766, Advanced Optical Imaging Technologies VI; 127660X (2023) https://doi.org/10.1117/12.2687083
Event: SPIE/COS Photonics Asia, 2023, Beijing, China

Conference Poster

Abstract

Frequency-shift super-resolution (SR) microscopy, such as structured illumination microscopy (SIM) and Fourier ptychographic microscopy (FPM), can break the diffraction limit for the imaging of both fluorescently labeled and label-free samples by transferring the high spatial-frequency information into the passband of microscope. However, conventional SIM microscopy systems tend to be bulky and expensive, which limits its applications in various fields. Therefore, we’ve developed some chip-based frequency-shift SR technologies, which is compatible with conventional microscope, and can be further designed for portable imaging systems such as smart phone and so on. We also developed a deep-learning based imaging method to improve the imaging speed of frequency-shift super-resolution microscopy, which enables low-cost and fast super-resolution imaging for real-time live cell biological studies.

Citation Download Citation

Qianwei Zhang, Feihong Lin, Xiaoyu Yang, Mingwei Tang, Qing Yang, and Xu Liu "Research on low-cost and fast frequency-shift super-resolution microscopic imaging", Proc. SPIE 12766, Advanced Optical Imaging Technologies VI, 127660X (29 November 2023); https://doi.org/10.1117/12.2687083

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available