Development of compact mouse retinal imaging system using spectral domain optical coherence tomography (SD-OCT) with a full field stimulation channel for recording fast optoretinograms (ORGs)

Hang Chan Jo; Ewelina Pijewska; Ratheesh K. Meleppat; Ravi S. Jonnal; Dae Yu Kim; Robert J. Zawadzki

doi:10.1117/12.3000280

12 March 2024 Development of compact mouse retinal imaging system using spectral domain optical coherence tomography (SD-OCT) with a full field stimulation channel for recording fast optoretinograms (ORGs)

Hang Chan Jo, Ewelina Pijewska, Ratheesh K. Meleppat, Ravi S. Jonnal, Dae Yu Kim, Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 12824, Ophthalmic Technologies XXXIV; 128240C (2024) https://doi.org/10.1117/12.3000280
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

While the a-wave of mouse electroretinogram (ERG) occurs within 50 milliseconds after exposure to light, the optoretinogram (ORG) slower than a 20Hz sampling rate could face limitations in observing immediate morphological changes from the retina. In this study, we develop a compact custom-built mouse ORG system based on spectral domain optical coherence tomography (SD-OCT) for 100Hz~1KHz B-scan rates comprised of 100 kHz A-scans. All the optics of the developed ORG system are designed on a 24 x 24 inches optical breadboard to move easily as well as to combine with the ERG system in a dark room. Without using a fundus camera, the OCT system provides en-face images from high-pass filtering and square of the OCT spectral signal for mouse retinal positioning in-vivo before acquiring ORG data. The 490nm LED for light stimulus is generated to make uniform illumination at the mouse retina using the Maxwellian view method. The common path of the OCT scanning light and the visible LED is built with achromatic doublet lens combinations based on optical simulation with Opticstudio^® . The developed compact ORG system can not only observe light-evoked responses with 1~10 milliseconds but also be used for the studies of correlations between ORG and ERG in the mouse retina.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Hang Chan Jo, Ewelina Pijewska, Ratheesh K. Meleppat, Ravi S. Jonnal, Dae Yu Kim, and Robert J. Zawadzki "Development of compact mouse retinal imaging system using spectral domain optical coherence tomography (SD-OCT) with a full field stimulation channel for recording fast optoretinograms (ORGs)", Proc. SPIE 12824, Ophthalmic Technologies XXXIV, 128240C (12 March 2024); https://doi.org/10.1117/12.3000280

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available