Experimental evidence on photobiomodulation induced by short wavelengths blue LED light

Giada Magni; Federica Cherchi; Martina Banchelli; Francesca Tatini; Patrizia Nardini; Anna Maria Pugliese; Daniele Guasti; Stefano Bacci; Lucia Cavigli; Roberto Pini; Francesca Rossi

doi:10.1117/12.3003669

12 March 2024 Experimental evidence on photobiomodulation induced by short wavelengths blue LED light

Giada Magni, Federica Cherchi, Martina Banchelli, Francesca Tatini, Patrizia Nardini, Anna Maria Pugliese, Daniele Guasti, Stefano Bacci, Lucia Cavigli, Roberto Pini, Francesca Rossi

Author Affiliations +

Proceedings Volume 12826, Mechanisms of Photobiomodulation Therapy XVIII; 1282604 (2024) https://doi.org/10.1117/12.3003669
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Skin serves as a vital component for thermoregulation and acts as a barrier against external threats. Wounds compromise the integrity of the skin and can lead to serious pathologies. Therefore, effective treatment of skin wounds is imperative, particularly in cases of chronic wounds or in individuals with comorbidities. Fibroblast cells play a crucial role in wound healing and represent a model for in vitro studies on photobiomodulation (PBM). The use of blue LED light in PBM has been shown to be effective in wound management, although the exact mechanism of action is still unclear. Several studies have investigated this, identifying specific target molecules, including Cytochrome C oxidase. Mitochondria appear to be a key target for blue light irradiation. To investigate this further, primary cultures of human dermal fibroblasts were established and a blue LED light device (410 to 430nm, 1W optical emission power) was used. A single application administered three doses of blue light (4, 21, 41 J/cm²). Mitochondrial morphology was observed before and after irradiation using electron microscopy. Subsequent studies will aim to determine if changes in morphology correspond to alterations in function. Previous in vitro findings indicated enhanced cell viability, proliferation, and outward currents at the lower fluence (4 J/cm²). Conversely, the higher dose (41 J/cm²) reduced cell viability. Here, we present preliminary results on the impact of 4 J/cm² blue LED light on outward currents in fibroblasts. Our results highlight the PBM properties of short-wavelength blue LED light.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Giada Magni, Federica Cherchi, Martina Banchelli, Francesca Tatini, Patrizia Nardini, Anna Maria Pugliese, Daniele Guasti, Stefano Bacci, Lucia Cavigli, Roberto Pini, and Francesca Rossi "Experimental evidence on photobiomodulation induced by short wavelengths blue LED light", Proc. SPIE 12826, Mechanisms of Photobiomodulation Therapy XVIII, 1282604 (12 March 2024); https://doi.org/10.1117/12.3003669

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