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The decay-kinetics of slow delayed luminescence from organic materials informs conditions such as the oxidative stress of the organism. And delayed luminescence can be induced by external stimulations not limited to photo-irradiation. Acquiring the decay-kinetics of delayed luminescence thus renders studying the response of the organism to external stress. At the absence of external stress causing delayed luminescence, however, organic materials may produce spontaneous ultraweak photon emission due to the residual oxygen demand. The value of steady-state ultraweak photon emission to studying the residual state of organism has been under debate. To better understand the dynamic and residual oxidative state of organism, it will be desirable to acquire both the stress-induced delayed luminescence and the steady-state photon emission. This, however, is challenging due to the significantly different scales of photon counts in the two types of photon-emission. The decay of delayed luminescence from an organism will reach a steady noisy baseline, which however may not be a random noise. Instead, the noisy baseline could be the spontaneous ultraweak photon-emission, which is challenging to measure by time-gated configuration for acquiring delayed luminescence, due to the weak signal overlaying with the noise. We demonstrate an irradiation-acquisition interleaved time-integrated imaging to acquire both the slow-decay kinetics of delayed luminescence and the steady-state ultraweak photon emission. The integration-mode acquisition enabled differentiating the steady-state ultraweak photon emission from the random baseline noise. And repetitive irradiation interleaved with integration at various durations of acquisition allowed the extraction of the decay-kinetics. Results from yeasts demonstrate the approach.
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