Structured illumination super-resolution imaging with lanthanide-doped upconversion nanoparticles

Baolei Liu; Yao Wang; Jiaqi Song; Menghan Tian; Ze Zheng; Xuchen Shan; Lei Ding; Chaohao Chen; Fan Wang

doi:10.1117/12.3015762

29 December 2023 Structured illumination super-resolution imaging with lanthanide-doped upconversion nanoparticles

Baolei Liu, Yao Wang, Jiaqi Song, Menghan Tian, Ze Zheng, Xuchen Shan, Lei Ding, Chaohao Chen, Fan Wang

Proceedings Volume 12975, 6th Optics Young Scientist Summit (OYSS 2023); 129750S (2023) https://doi.org/10.1117/12.3015762
Event: 6th Optics Young Scientist Summit, 2023, Changsha, China

Abstract

Super-resolution microscopy provides a high spatial resolution that is beyond the diffraction barrier and can visualize nano-sized structures and interactions in biological and material study. In recent years, lanthanide-doped upconversion nanoparticles (UCNPs) that can upconvert the near-infrared (NIR) excitation photons to visible emission photons, have been developing as a kind of novel nanoprobes for bioimaging. Here we report the recently developed NIR superresolution imaging techniques by exploring the nonlinear fluorescence responses in UCNPs. Upconversion Nonlinear Structured Illumination Microscopy (U-NSIM) employes nonlinear fluorescence, along with NIR excitation and emission light, to deliver rapid frame rates and high-resolution capabilities, enabling in-depth super-resolution imaging. The tunability of lifetime in UCNPs is also introduced to develop the multiplexed super-resolution imaging with lifetime-engineered nanoprobes. By detecting two emission channels with different nonlinear fluorescence responses, a single doughnut illumination beam is used to scan the sample to generate a Gaussian-like emission point spread function (PSF) and a doughnut-emission PSF simultaneously, which can be fused by algorithms to an optimized super-resolution nanoscopy. These upconversion super-resolution imaging techniques provide new strategies to develop deep-tissue and multiplexed super-resolution imaging and also help to achieve it in a simple optical scheme.

(2023) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Baolei Liu, Yao Wang, Jiaqi Song, Menghan Tian, Ze Zheng, Xuchen Shan, Lei Ding, Chaohao Chen, and Fan Wang "Structured illumination super-resolution imaging with lanthanide-doped upconversion nanoparticles", Proc. SPIE 12975, 6th Optics Young Scientist Summit (OYSS 2023), 129750S (29 December 2023); https://doi.org/10.1117/12.3015762

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KEYWORDS

Super resolution

Near infrared

Upconversion

Light sources and illumination

Nanoparticles

Biological imaging

Microscopy

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