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Bacterial biofilms exist as aggregates of bacterial cells enclosed in a polysaccharide matrix, offering them resistance to various mechanical, chemical, antimicrobial, and environmental stress. The formation of biofilm on solid substrates occurs through a series of steps. It is essential to understand the stage of biofilm formation for several biomedical applications, such as assessing the coating efficacy for hard tissue implants, devising treatment strategies to inhibit biofilm formation in thin tissues, and building biocompatible diagnostic devices. In this work, we propose Laser Speckle Image Analysis to evaluate the bacterial biofilm formation stages with the help of different parameters extracted from the laser speckle image. These parameters give insight into the concentration and orientation changes of bacteria through the different stages of biofilm. The formation of biofilm on polypropylene substrate is evaluated and validated with optical microscopy images.
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