Raman glycoprobes for the detection of pathogenic fungi

Keela Kessie; Ryan Muddiman; Rachel Keenan Dillon; Timothy McNamara; Kevin Kavanagh; Bryan Hennelly; Trinidad Velasco-Torrijos

doi:10.1117/12.3022511

20 June 2024 Raman glycoprobes for the detection of pathogenic fungi

Keela Kessie, Ryan Muddiman, Rachel Keenan Dillon, Timothy McNamara, Kevin Kavanagh, Bryan Hennelly, Trinidad Velasco-Torrijos

Author Affiliations +

Proceedings Volume 13006, Biomedical Spectroscopy, Microscopy, and Imaging III; 130061I (2024) https://doi.org/10.1117/12.3022511
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Antimicrobial Resistant (AMR) fungal pathogens do not respond to conventional treatments, causing lethal infections, especially in immunocompromised people. The urgent need for fast, reliable, and highly specific diagnostic methods to control this silent pandemic is evident. Raman Spectroscopy methods have great potential for the detection and identification of microbial pathogens, either label- free or using specific Raman tags and probes. We are developing carbohydrate-based Raman probes aiming to achieve selective pathogen detection inspired by the first steps of infection, during which pathogens adhere to the surface of host cells via carbohydrateprotein interactions. Previously, our group identified an aromatic-core divalent galactoside, that mimics host cell carbohydrates and recognizes Candida albicans, a critical priority fungal pathogen. We have synthesized thiol-bearing derivatives of this compound, which are attached to the surface of gold nanoparticles to create novel Raman glycoprobes capable of binding C. albicans. These novel glycoprobes will be studied for the capture, detection and chemical imaging of fungal pathogens, such as C. albicans and Aspergillus fumigatus using coherent Raman spectroscopic techniques. Ultimately, we aim to optimize this approach for the capture, imaging and identification of multiple pathogens in a biological sample. Herein, we present our current progress.

Citation Download Citation

Keela Kessie, Ryan Muddiman, Rachel Keenan Dillon, Timothy McNamara, Kevin Kavanagh, Bryan Hennelly, and Trinidad Velasco-Torrijos "Raman glycoprobes for the detection of pathogenic fungi", Proc. SPIE 13006, Biomedical Spectroscopy, Microscopy, and Imaging III, 130061I (20 June 2024); https://doi.org/10.1117/12.3022511

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