In-vivo and in-situ particle diagnostics

Clarice M. Yentsch; Terry L. Cucci

doi:10.1117/12.21442

1 September 1990 In-vivo and in-situ particle diagnostics

Clarice M. Yentsch, Terry L. Cucci

Proceedings Volume 1302, Ocean Optics X; (1990) https://doi.org/10.1117/12.21442
Event: 1990 Technical Symposium on Optics, Electro-Optics, and Sensors, 1990, Orlando, FL, United States

Abstract

Particles from both natural populations and culture mixtures are categorized into microautotroph and detritus/microheterotroph components based on size and chlorophyll autofluorescence. Subcomponents ofmicroautotroph pigment groups are possible by the addition of phycoerythrin autofluorescence measurements. Subcomponents of microautotrophs as "live" and "dead" cells are determined by the addition of the metabolic activity stain fluorescein diacetate (FDA). The detritus/microheterotroph fraction includes green-fluorescing cells, abundant in the Gulf of California, detritus, and bacteria. The relative contributions of icroautotrophs, microheterotrophs and detritus mustbe discerned in order to fully interpret beam attenuation and changes in beam attenuation.

Citation Download Citation

Clarice M. Yentsch and Terry L. Cucci "In-vivo and in-situ particle diagnostics", Proc. SPIE 1302, Ocean Optics X, (1 September 1990); https://doi.org/10.1117/12.21442

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