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The work described in this paper built a holographic microscopic optical tweezers system in the laboratory and wrote an algorithm for generating phase maps based on diffraction-computed holography, thus break through the limitation that the traditional optical tweezers system can only capture the target at the center of the optical path and realizing free manipulation of targeting plankton in the field of view. Meanwhile, this paper also miniaturizes the laboratory system and completes the design including the underwater chamber and related accessories so that the system can work well underwater. The experiments show that the system can stabilize and image plankton samples well in both static water and flow field, which can meet the requirements of underwater in-situ experiments, indicating that the method has great practical value and application prospects in solving the problem of high-quality in-situ microscopic observation. |
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Optical tweezers
Detection and tracking algorithms
Imaging systems
Particles
Holography
Ocean optics
Holograms
