1 July 1990 Three-dimensional imaging in microscopy
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Proceedings Volume 1319, Optics in Complex Systems; (1990); doi: 10.1117/12.34812
Event: 15th International Optics in Complex Systems, 1990, Garmisch, Germany
Abstract
A theory of 3D- imaging of a thick object with a depth h (Fig.l ) is developed for vertical Illumination microscopy (Fig2). The amount as well as the phase of the Fourier spectrum which corresponds to the 3D- object is discussed in detaiL Fig3 shows the amount tT(01 of the Fourier spectrum for different object depth. ii (V I has zercs In the case of planar object (h0) . As the thickness of the object is increased an enhancement of the spatial frequencies and a shift of the Ninhia occurs. The zeri of the planar object spectrum are lsed.
© (1990) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
S. Kessler, Rosemarie Hild, G. Nitzche, Jork Hebenstreit, "Three-dimensional imaging in microscopy", Proc. SPIE 1319, Optics in Complex Systems, (1 July 1990); doi: 10.1117/12.34812; https://doi.org/10.1117/12.34812
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