The binding of two isomeric 2-[(chysenylmethyl)amino]-2-methyl- 1 ,3-propanediols
(AMAPs) to the biopolymer poly(dG-dC)•poly(dG-dC) was studied using Raman spectroscopy.
The Raman spectra of the bound AMAPs were obtained by subtracting the Raman spectrum of the
biopolymer in buffered aqueous solution from those recorded for the AMAP-DNA binary
complexes. The difference method applied here has been used in other studies to obtain spectra
of small molecules bound to macromolecules of biological origin (Yue, K.T. et al., J. Raman
Spectrosc., 20, 541-545, 1989, and references therein). Due to the extremely low number of
Raman active lines observed in the spectra of these AMAPs, it was also possible to study
perturbations in the Raman spectrum of the complexed biopolymer.
Spectroscopic data indicated that both AMAPs intercalate with DNA, in agreement with
viscometric measurements for these compounds; however, some differences were observed in the
Raman spectra of DNA complexed with these drugs, suggesting that the specific AMAP-DNA
binding interactions are different.