Nuclear recognition in images of fluorescent-stained cell monolayers

Jeffrey H. Price M.D.; David A. Gough

doi:10.1117/12.23541

1 November 1990 Nuclear recognition in images of fluorescent-stained cell monolayers

Jeffrey H. Price M.D., David A. Gough

Author Affiliations +

Proceedings Volume 1349, Applications of Digital Image Processing XIII; (1990) https://doi.org/10.1117/12.23541
Event: 34th Annual International Technical Symposium on Optical and Optoelectronic Applied Science and Engineering, 1990, San Diego, CA, United States

Abstract

A method for reliable recognition of fluorescent stained cell monolayers has been developed. This method utilizes convolution filtering and object intensity dependent thresholding to achieve accurate recognition. These operations are performed rapidly by dedicated image processing hardware. Details of the transfer of image information into efficient representation in computer memory are presented. These methods are utilized in conjunction with a computer-controlled microscope stage to scan the specimen field-by-field. An example of the measurements possible with this system is provided by presentation of a DNA content histogram from analysis of over 50 Because this system is capable of analyzing attached cells it offers an alternative to flow cytomeiry which requires that cells be in suspension.

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Jeffrey H. Price M.D. and David A. Gough "Nuclear recognition in images of fluorescent-stained cell monolayers", Proc. SPIE 1349, Applications of Digital Image Processing XIII, (1 November 1990); https://doi.org/10.1117/12.23541

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