Computer aided 3D localization of Chromosome 1 within nuclei of human sperm cells

Thomas Leemann; Vito Baggiolini; Heinrich Walt; Patricia Emmerich; Max Anliker

doi:10.1117/12.2294321

1 August 1990 Computer aided 3D localization of Chromosome 1 within nuclei of human sperm cells

Thomas Leemann, Vito Baggiolini, Heinrich Walt, Patricia Emmerich, Max Anliker

Proceedings Volume 1395, Close-Range Photogrammetry Meets Machine Vision; 139526 (1990) https://doi.org/10.1117/12.2294321
Event: Close-Range Photogrammetry Meets Machine Vision, 1990, Zurich, Switzerland

Abstract

Conventional cellular and histological analyses are generally based on planar images. New preparation techniques in the field of immunohistochemistry and in situ hybridization demand a more sophisticated analytical approach. Our method to detect irregularities in the number of chromosomes present in interphase nuclei of normal and neoplastic cells and to characterize aberrations in the structure of these chromosomes makes use of a combination of confocal laser scanning microscopy (CLSM) and 3-D imaging. In this paper we describe a computer-assisted setup designed to localize in situ hybridized chromosome 1 of human sperm cells. In order to synthesize a 3-D image of the structures of interest, the stack of slice images generated by the CLSM is processed appropriately with the aid of a parallel distributed computer architecture based on transputers. The resulting images or particular aspects thereof are visualized on a high resolution display and related to new information in reproductive biology.

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Thomas Leemann, Vito Baggiolini, Heinrich Walt, Patricia Emmerich, and Max Anliker "Computer aided 3D localization of Chromosome 1 within nuclei of human sperm cells", Proc. SPIE 1395, Close-Range Photogrammetry Meets Machine Vision, 139526 (1 August 1990); https://doi.org/10.1117/12.2294321

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KEYWORDS

Image processing

Control systems

Machine vision

Photogrammetry

Confocal microscopy

Microscopes

Visualization

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