1 April 1991 Liquid-crystal-television-based optical-digital processor for measurement of shortening velocity in single rat heart cells
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Proceedings Volume 1398, CAN-AM Eastern '90; (1991) https://doi.org/10.1117/12.47793
Event: CAN-AM Eastern '90: 1st Canadian-American Eastern Regional Conference on Applications of Optical Engineering, 1990, Rochester, NY, United States
Abstract
Cardiac cells exhibit rhythmic contractions when electrically paced and maintain striation patterns throughout the contraction-relaxation cycle similar to one-dimensional diffraction grating that changes in spatial frequency. The current study demonstrates the application of an on-line hybrid optical-digital processor with liquid crystal television (LCTV) acting as a spatial light modulator(SLM) to monitor the dynamics of contraction of single cardiac cells in real-time . The processor which is interfaced with a phase contrast microscope performs the Fourier transformation of the cell''s striated image optically and records the Fourier spectra digitally using a charge coupled device (CCD) camera. A series of digitized images of the Fourier spectra each of duration of 16 . 67 msec (non-interlaced frames) is captured during one contraction cycle (''4000 msec). The sarcomere length at each time point and contraction velocities are calculated. The overall system is initially calibrated using a high-resolution Ealing target. Experimental results demonstrate the improvement of contrast by about 300 using the LCTV and the optical-digital processor can faithfully track sarcomere mechanics. 1 .
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rao V. Yelamarty, Rao V. Yelamarty, Francis T. S. Yu, Francis T. S. Yu, Russell L. Moore, Russell L. Moore, Joseph Y. Cheung, Joseph Y. Cheung, } "Liquid-crystal-television-based optical-digital processor for measurement of shortening velocity in single rat heart cells", Proc. SPIE 1398, CAN-AM Eastern '90, (1 April 1991); doi: 10.1117/12.47793; https://doi.org/10.1117/12.47793
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