1 May 1991 DNA photoproducts formed using high-intensity 532-nm laser radiation
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Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57311
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation
Abstract
Cyclobutylpyrimidine dimers were detected in double-stranded plasmid DNA after exposure to 532-nm 28-ps pulses at intensities (1-8 GW/cm2) below the threshold for optical breakdown. Formation of these photoproducts was attributed to simultaneous two-photon absorption by nucleotides in DNA because the rate of dimer formation was dependent on the square of the intensity and because an equivalent number of photons at 514 nm delivered at low intensity did not produce dimers. The average two-photon cross-section at 532 rim for absorption by a nucleotide was found to be 0. 50 ( 0. 02) x l052 cm4sec'' photon* Intensities of 532-rim radiation above the threshold for optical breakdown caused DNA strand breaks by radical and mechanical mechanisms. 1 .
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Irene E. Kochevar, Irene E. Kochevar, Yaron Hefetz, Yaron Hefetz, D. A. Dunn, D. A. Dunn, T. F. Deutsch, T. F. Deutsch, Lisa A. Buckley, Lisa A. Buckley, Franz Hillenkamp, Franz Hillenkamp, } "DNA photoproducts formed using high-intensity 532-nm laser radiation", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57311; https://doi.org/10.1117/12.57311
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