Intracellular location, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins

Herbert Schneckenburger; Harold K. Seidlitz; Jurina M. Wessels; Angelika C. Rueck

doi:10.1117/12.57320

1 May 1991 Intracellular location, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins

Herbert Schneckenburger, Harold K. Seidlitz, Jurina M. Wessels, Angelika C. Rueck

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57320
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Porphyrin monomers dimers and aggregates with various tumour-localizing and photosensitizing properties can be differentiated on the basis of their fluorescence lifetimes. These individual components are shown to possess different degrees of polarization. This fact could be used for their microscopic imaging as well as for measurements of porphyrin coupling within cells and tissues. The spectral bands around 635 nm and 675 nm were correlated with the emission of porphyrin monomers and aggregates respectively. The formation of photo-protoporphyrin was not significant during the light-induced porphyrin reaction.

Citation Download Citation

Herbert Schneckenburger, Harold K. Seidlitz, Jurina M. Wessels, and Angelika C. Rueck "Intracellular location, picosecond kinetics, and light-induced reactions of photosensitizing porphyrins", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57320

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