Studies of the excited states of biological systems using UV-excited resonance Raman and picosecond transient Raman spectroscopy

Terry L. Gustafson; Koichi Iwata; William L. Weaver; Lisa A. Huston; Ronda L. Benson

doi:10.1117/12.57303

1 May 1991 Studies of the excited states of biological systems using UV-excited resonance Raman and picosecond transient Raman spectroscopy

Terry L. Gustafson, Koichi Iwata, William L. Weaver, Lisa A. Huston, Ronda L. Benson

Author Affiliations +

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57303
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Ground state resonance Raman spectroscopy and picosecond transient Raman spectroscopy provide similar information. The former method superimposes excited state structural information on the ground state vibrational spectrum while the latter method is a direct probe of the excited state vibrational spectrum. When the two methods are taken together the resonance Raman spectrum provides a means for correlating vibrational bands in the ground state spectrum with bands observed in the excited state transient spectra. We are using a MHz amplified synchronously pumped dye laser as the source for our experiments. We demonstrate recent improvements in the laser system that increase the power and extend the wavelength coverage. We use the source to study the vibrational spectra of dilute solutions of biological molecules and to study the structure of the excited state of trans-stilbene in several solvents. 1.

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Terry L. Gustafson, Koichi Iwata, William L. Weaver, Lisa A. Huston, and Ronda L. Benson "Studies of the excited states of biological systems using UV-excited resonance Raman and picosecond transient Raman spectroscopy", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57303

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