Detection of atheroma using Photofrin II and laser-induced fluorescence spectroscopy

Sandor G. Vari M.D.; Theodore G. Papazoglou; Maurits J. van der Veen; Thanassis Papaioannou; Michael C. Fishbein M.D.; Mudjianto Chandra M.D.; Clain Beeder; Wei-Qiang Shi; Warren S. Grundfest M.D.

doi:10.1117/12.44081

1 June 1991 Detection of atheroma using Photofrin II^r and laser-induced fluorescence spectroscopy

Sandor G. Vari M.D., Theodore G. Papazoglou, Maurits J. van der Veen, Thanassis Papaioannou, Michael C. Fishbein M.D., Mudjianto Chandra M.D., Clain Beeder, Wei-Qiang Shi, Warren S. Grundfest M.D.

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Proceedings Volume 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques; (1991) https://doi.org/10.1117/12.44081
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The goal of this study was to investigate laser induced fluorescence spectroscopy (LIFS) as a method of localization of atherosclerotic lesions not visible by angiography using Photofrin IIr enhanced fluorescence. Twenty-four New Zealand White rabbits divided into six groups varying in type of arterial wall lesion and Photofrin IIr administration time (i.v.) were used. Aortic wall fluorescence signals were acquired from the aortic arch to iliac bifurcation. The output of a He-Cd laser (442 nm, 17 mW) was directed at the arterial wall through a 400 micron fiber. The fluorescence signal created in the arterial wall was collected via the same fiber and analyzed by an optical multi-channel analyzer (OMA). The ratio of fluorescence intensities at 630 nm (Photofrin IIr) and 540 nm (autofluorescence of artery wall) was analyzed (I630nm/I540nm). Intensity ratio values 24 hours after administration of Photofrin IIr were found to be as follows: in normal artery wall of 0.30 +/- 0.14 (n equals 3), in mechanically damaged wall of 0.91 +/- 0.65 (n equals 2) and, in atheromatous tissue, 0.88 +/- 0.54 (n equals 4). The intensity ratio of atheromatous tissue without Photofrin IIr was 0.23 +/- 0.04 (n equals 7). These results suggest that the use of Photofrin IIr allows in vivo atheroma detection by LIFS because of its ability to accumulate in atheroma. In addition, accumulation of Photofrin IIr was found in artery walls traumatized by balloon catheter intervention. Using this method, a catheter-based LIFS system may be developed for atheroma detection.

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Sandor G. Vari M.D., Theodore G. Papazoglou, Maurits J. van der Veen, Thanassis Papaioannou, Michael C. Fishbein M.D., Mudjianto Chandra M.D., Clain Beeder, Wei-Qiang Shi, and Warren S. Grundfest M.D. "Detection of atheroma using Photofrin II^r and laser-induced fluorescence spectroscopy", Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); https://doi.org/10.1117/12.44081

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KEYWORDS

Laser induced fluorescence

Luminescence

Arteries

Tissue optics

Fluorescence spectroscopy

Spectroscopy

Angiography

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