Intraoperative metastases detection by laser-induced fluorescence spectroscopy

Sandor G. Vari M.D.; Theodore G. Papazoglou; Maurits J. van der Veen; Michael C. Fishbein M.D.; J. D. Young; Mudjianto Chandra M.D.; Thanassis Papaioannou; Clain Beeder; Wei-Qiang Shi; Warren S. Grundfest M.D.

doi:10.1117/12.44051

1 June 1991 Intraoperative metastases detection by laser-induced fluorescence spectroscopy

Sandor G. Vari M.D., Theodore G. Papazoglou, Maurits J. van der Veen, Michael C. Fishbein M.D., J. D. Young, Mudjianto Chandra M.D., Thanassis Papaioannou, Clain Beeder, Wei-Qiang Shi, Warren S. Grundfest M.D.

Author Affiliations +

Proceedings Volume 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques; (1991) https://doi.org/10.1117/12.44051
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

The authors studied the ability of Laser Induced Fluorescence Spectroscopy (LIFS) for the intraoperative identification of metastases using a photosensitizing agent Photofrin IIr to enhance spectroscopic detection. A He-Cd laser source (442 nm) was used to produce low-power illumination of tissue via a hand-held 400 micrometers fiberoptic probe. Through the same fiber, reflected and emitted light was returned to an optical multi-channel analyzer (OMA III) for analysis. Spectroscopic signals were displayed on a screen for immediate examination. Lobund Wistar rats, inoculated with Pollard rat adenocarcinoma cells, were used as an animal model. Photofrin IIr was administered intraperitoneal 24 or 48 hours prior to surgical exploration in doses varying from 0.75-7.5 mg/kg. Metastases detection was performed during abdominal exploration directed to ipsilateral and contralateral inguinal, iliac, para-aortic and renal lymph nodes. Nineteen tissue samples, identified as abnormal by LIFS, were removed for histologic analysis; 11 of these samples were larger than 5mm and histologic examination revealed malignancy in all cases. While LIFS signals showed malignancy in 8 tissue samples with dimensions less than 5mm, histology confirmed this in only 3. However, serial histologic sections were not performed. From the initial results, it was concluded that LIFS detection of malignant tissue is feasible and enhanced by the addition of Photofrin IIr. LIFS may be a promising technique for the intraoperative detection of primary malignant and metastatic tissue.

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Sandor G. Vari M.D., Theodore G. Papazoglou, Maurits J. van der Veen, Michael C. Fishbein M.D., J. D. Young, Mudjianto Chandra M.D., Thanassis Papaioannou, Clain Beeder, Wei-Qiang Shi, and Warren S. Grundfest M.D. "Intraoperative metastases detection by laser-induced fluorescence spectroscopy", Proc. SPIE 1426, Optical Methods for Tumor Treatment and Early Diagnosis: Mechanisms and Techniques, (1 June 1991); https://doi.org/10.1117/12.44051

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