1 May 1991 HIV detection by in-situ hybridization based on confocal reflected light microscopy
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Proceedings Volume 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences; (1991) https://doi.org/10.1117/12.44144
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States
Abstract
Elucidation of the pathogenesis of AIDS is confounded by the finding that few actively infected CD4+ cells (1 in 104-105) can be detected in the peripheral blood, even though there is dramatic depletion (often >90%) of CD4+ cells as the disease progresses. A sensitive, 35S-based human immunodeficiency virus (HIV) mRNA in situ hybridization technique was coupled with a new detection method, confocal laser scanning microscopy, to examine transcriptionally active HIV-infected cells from individuals at different disease stages. An algorithm for image segmentation and analysis has been developed to determine the proportion of HIV-positive cells. Data obtained using this improved detection method suggest that there are more HIV mRNA-producing cells in HIV-infected individuals than previously thought, based on other detection methods.
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Louis C. Smith, Louis C. Smith, Zeljko Jericevic, Zeljko Jericevic, Roland Cuellar, Roland Cuellar, Stephen W. Paddock, Stephen W. Paddock, Dorothy E. Lewis, Dorothy E. Lewis, } "HIV detection by in-situ hybridization based on confocal reflected light microscopy", Proc. SPIE 1428, Three-Dimensional Bioimaging Systems and Lasers in the Neurosciences, (1 May 1991); doi: 10.1117/12.44144; https://doi.org/10.1117/12.44144
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