Nanosecond time-resolved natural and magnetic circular dichroism spectroscopy of protein dynamics

Robert A. Goldbeck; Sophie Bjorling; David S. Kliger

doi:10.1117/12.44205

1 June 1991 Nanosecond time-resolved natural and magnetic circular dichroism spectroscopy of protein dynamics

Robert A. Goldbeck, Sophie Bjorling, David S. Kliger

Proceedings Volume 1432, Biomolecular Spectroscopy II; (1991) https://doi.org/10.1117/12.44205
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States

Abstract

A recently developed ellipsometric technique for the nanosecond time-resolved measurement of natural and magnetic circular dichroism (TRCD and TRMCD) spectra is discussed and applications to dynamical studies of biologically important molecules, principally hemeproteins, are presented. Nanosecond TRCD spectra of the Soret band of hemoglobin and myoglobin have been measured after photolysis of the carbonmonoxy complex. The TRCD of the photoreduction of metmyoglobin has also been studied. Nanosecond TRMCD spectroscopy of cytochrome c oxidase (cytochrome aa3) gives information about the spin state of the iron in the heme a3 transient intermediate formed after photolysis of the CO complex. Applications of TRMCD spectroscopy to cytochromes ba3 and c3 are also discussed, as are TRCD studies of phytochrome proteins. Recent progress in characterizing and eliminating spectral artifacts arising from photoselection in photolyzed proteins is summarized.

Citation Download Citation

Robert A. Goldbeck, Sophie Bjorling, and David S. Kliger "Nanosecond time-resolved natural and magnetic circular dichroism spectroscopy of protein dynamics", Proc. SPIE 1432, Biomolecular Spectroscopy II, (1 June 1991); https://doi.org/10.1117/12.44205

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available