1 June 1991 Use of fluorescence spectroscopy to elucidate structural features of the nicotinic acetylcholine receptor
Author Affiliations +
Proceedings Volume 1432, Biomolecular Spectroscopy II; (1991) https://doi.org/10.1117/12.44210
Event: Optics, Electro-Optics, and Laser Applications in Science and Engineering, 1991, Los Angeles, CA, United States
Abstract
The authors review three examples of how fluorescence spectroscopic techniques have been employed to elucidate structural features of the nicotinic acetylcholine receptor from the electric organ of Torpedo californica. The instrumentation and most relevant fluorescence methods utilized for these studies are briefly discussed. The examples reviewed are as follows: (1) the measurement of the average distance between the two acetylcholine binding sites on the receptor and the noncompetitive inhibitor binding site in the central ion channel, (2) the measurement of distance between fluorescently labeled snake (alpha) -toxins bound to the same membrane-associated receptor oligomer and bound to the adjacent receptor oligomers, and (3) the relative orientation of snake (alpha) -toxin with respect to the central ion channel and the plane of the lipid membrane into which the receptor is embedded.
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
David Allen Johnson, David Allen Johnson, C. Fernando Valenzuela, C. Fernando Valenzuela, } "Use of fluorescence spectroscopy to elucidate structural features of the nicotinic acetylcholine receptor", Proc. SPIE 1432, Biomolecular Spectroscopy II, (1 June 1991); doi: 10.1117/12.44210; https://doi.org/10.1117/12.44210
PROCEEDINGS
9 PAGES


SHARE
Back to Top