1 December 1991 Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera
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Proceedings Volume 1439, International Conference on Scientific Optical Imaging; (1991) https://doi.org/10.1117/12.50457
Event: International Conference on Scientific Optical Imaging, 1990, Georgetown, Cayman Islands
Abstract
Biological samples have been imaged using microscopes equipped with slow-scan CCD cameras. Examples are presented of studies based on the detection of light emission signals in the form of fluorescence and phosphorescence. They include applications in the field of cell biology: (a) replication and topology of mammalian cell nuclei; (b) cytogenetic analysis of human metaphase chromosomes; and (c) time-resolved measurements of DNA-binding dyes in cells and on isolated chromosomes, as well as of mammalian cell surface antigens, using the phosphorescence of acridine orange and fluorescence resonance energy transfer of labeled lectins, respectively.
© (1991) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas M. Jovin, Thomas M. Jovin, Donna J. Arndt-Jovin, Donna J. Arndt-Jovin, } "Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera", Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); doi: 10.1117/12.50457; https://doi.org/10.1117/12.50457
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