Performance of a thinned back-illuminated CCD coupled to a confocal microscope for low-light-level fluorescence imaging

Barry R. Masters

doi:10.1117/12.45315

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1 July 1991 Performance of a thinned back-illuminated CCD coupled to a confocal microscope for low-light-level fluorescence imaging

Barry R. Masters

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Proceedings Volume 1447, Charge-Coupled Devices and Solid State Optical Sensors II; (1991) https://doi.org/10.1117/12.45315
Event: Medical Imaging '91, 1991, San Jose, CA, United States

Abstract

This paper describes the results of coupling a Tektronix TK512 CCD imager to a Technical Instruments K2Bio Nipkow disk real-time confocal microscope. The characteristics of the selected TK512 CCD imager are as follows: the chip is a thinned, back-illuminated device which has an anti-reflection coating. The quantum efficiency of the device is 86.4 at 400 nm. The CCD imager is a TK512, silicon CCD which is sensitive from the UV to the near-IR. The sensor is a full-frame area imager, with CCDs fabricated using a buried channel, three-level polysilicon gate process with very high charge-transfer efficiency ($2 0.9999) and low dark current. Low noise on-chip amplifiers provide and interface to external preamplifiers with readout noise typically

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Barry R. Masters "Performance of a thinned back-illuminated CCD coupled to a confocal microscope for low-light-level fluorescence imaging", Proc. SPIE 1447, Charge-Coupled Devices and Solid State Optical Sensors II, (1 July 1991); https://doi.org/10.1117/12.45315

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