This paper presents a computerized technique for quantitative analysis of the movement characteristics of spermatozoa. Stored video images of spermatozoa are digitized at a fixed time interval. The digital images are stored as a sequence of frames in a microcomputer. The analysis of the sequence comprises two main tasks: finding the location of the centroid for each sperm and tracking them over the entire sequences. Information from the motion of each moving cell will be used for tracking. Experimental results are presented to show the merits of the proposed algorithm for tracking.